Studies of Bacillus subtilis NAP1 to degrade BOD, COD, TSS, and pH: The indigenous bacteria in Indonesia batik wastewater

Abstract

Batik is an industry that is quite popular in Indonesia. Batik has been declared as one of Indonesia’s cultural heritage and is recognized by UNESCO. Batik production is recorded to always increase every year. Unfortunately, the batik industry in Indonesia is dominated by many small industries with limited fund management. This makes the environmental aspects and waste disposal neglected. Through this research, study and isolation were carried out to explore the potential of indigenous bacterial isolates that can biodegrade dyes in batik along with other physical parameters of waste. A sample that contained wastewater and sediment from batik industry are collected and cultured in 1000 ml Busnall Hass medium which olive oil-enriched and put at the shaker condition at 150 rpm until 6 days at 30°C. Identification of the isolates examined for their morphological, physiological and biochemistry test. Bacillus subtilis is one of the indigenous isolates from this research. This study aimed to determine the ability of B. subtilis to reduce BOD, COD, TSS, and pH in batik waste and present the results of the DNA analysis of B. subtilis. Based on the research that has been done, it can be concluded that the molecular identification of bacteria through analysis of the 16S rDNA gene fragment sequence, the bacteria that play a role in the degradation of dyes is Bacillus subtilis strain NAP1 with an average similarity level of 93%. B. subtilis NAP1. B. subtilis NAP1 isolate offers the potential for future bioremediation of batik wastewater.