Studies of 15N-labeled lysine in colostomized laying hens. 3. 15N incorporation into the amino acids of the follicle, residual ovary and oviduct

Abstract

In a metabolism experiments with 15N-labelled lysine 3 colostomized laying hybrids received over 4 days 0.2% L-Lysine with 48 atom-% 15N-excess (15N') in addition to a ration conventionally produced and, subsequent to this, unlabelled lysine for four days. At the end of the experiment the hens were butchered: individual organs and tissues were prepared for 15N-analysis. The incorporation of the lysine-15N' into the further amino acids in the follicles, the ovary and the oviduct is described. The atom-% 15N' of the complete range of amino acids was analysed in the individual follicles. Various levels of heavy nitrogen could be detected in all essential and non-essential amino acids. Of the total amount of 15N' detected in the follicles 64.0% 65.0% and 61.2% resp. could be detected in lysine and 25.2%, 25.4%, 28.7% resp. in the other amino acids (hens 1 to 3). In the ovary on average 61.6% and on the oviduct 54.2% of the respective 15N'-amount was detected in lysine. In the ovary 10.9% and in the oviduct 8.4% 15N' of the total 15N' of these samples were incorporated into the arginine molecules.