Abstract
The determination of l-ascorbic, and dehydro-l-ascorbic plus diketo-l-gulonic acids in microgram quantities of tissue was described. The procedure used is an adaptation to the micro scale of macro methods based on spectrophotometric measurements of reactions involving 2,6-dichlorophenol indophenol and 2,4-dinitrophenylhydrazine, and it is well suited to serial analyses on large numbers of samples because of its relatively great simplicity and speed. The standard deviation in the ascorbic acid method is about 2% corresponding to about 3 mµg. of ascorbic acid for the equipment used. With pipettes and cuvettes of smaller capacity a reduction in the latter value by about one-third can be obtained without inconvenience.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
