Abstract
Publisher Summary This chapter discusses the microfiltration as a means of separating free antigen from antigen–antibody complexes in immunoassay. In immunoassay techniques, efficient separation of free antigen from the antigen–antibody complexes is essential for reproducible results and maximum sensitivity. Microfiltration, under suitable conditions, gives efficient separation and ease of handling large or small numbers of samples. The technique of microfiltration is more adaptable to mechanization than centrifugation. Microfiltration, using glass fiber filter disks, is an efficient alternative to centrifugation for the separation of free antigen from antigen–antibody complexes. When small numbers of samples are involved in manual methods or for mechanized apparatus, microfiltration has advantages over centrifugation. For immunoassay techniques, cellulose acetate filters offer no advantage over glass fiber filter paper, and the slow flow rate and the handling problems of cellulose acetate, when compared with glass fiber, make it less suitable for use when large numbers of samples are involved. The retention characteristics of GF/B filter paper are adequate for most immunoassay techniques. However, in receptor assays, TM, the particle sizes are close to the minimum retention size of the GF/B filter paper.
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