Abstract
Diabetes is marked by hyperglicemia with severe symptoms included polydipsi, polyuri, polyphagia,weight loss, and decreased of insulin sensitivity index specially at type 2 diabetes mellitus. The objective of this research was to analyze the characteristics of diabetes mellitus in rat (Rattus novergicus) which induced by dexamethason. A total of 24 rats were divided into four groups which divided as follow: (1) aquabidest subcutaneously (K0), (2) subcutaneous dexamethasone 1 mg/kgBW daily (K1), (3) subcutaneous dexamethasone 2.5 mg/kgBW daily (K2) and subcutaneous dexamethasone 5 mg/kgBW daily (K3). The data of fasting blood glucose, fasting blood insulin, body weight and feed intake, urine volume and water intake collected at day 0, 5, 10 and 15 (five days after the last treatment). The result showed that there was a significant elevation of glucose level followed by significant decreased of insulin sensitivity index and the body weight of rats in group wich induced by dexamethasone but there was no change in feed intake, water intake, volume of urine and fasting blood insulin before and after the treatment. There was insignificant different of total beta pancreatic islet between treatment and control grup. Induction of dexamethasone in mice causes reversible hyperglycemia and has not met the characterisics of either type 1 or 2 diabetes mellitus models. The profile of dexamethasone induced diabetes model has their own spesific type.
Highlights
Diabetes mellitus (DM) merupakan suatu penyakit metabolik multisistem dengan ciri hiperglikemia akibat kelainan sekresi insulin, kerja insulin, atau kedua-duanya (Dipiro 2005)
Diabetes is marked by hyperglicemia with severe symptoms included polydipsi, polyuri, polyphagia, weight loss, and decreased of insulin sensitivity index specially at type 2 diabetes mellitus
The objective of this research was to analyze the characteristics of diabetes mellitus in rat (Rattus novergicus) which induced by dexamethason
Summary
Penelitian ini menggunakan rancangan acak lengkap pola faktorial dengan objek penelitian tikus putih jantan dengan bobot badan ± 400 g. Awal sebelum perlakuan (T0), diambil data pengukuran klinis, kadar glukosa darah dan kadar insulin sebagai baseline (P0). Sebagian tikus pada T2 dikorbankan nyawanya dan dilakukan pemeriksaan sel beta pankreas dengan pewarnaan imunohistokimia. Dilihat kestabilan diabetes yang ditimbulkan oleh induksi deksametason dengan mengambil data pengukuran dari sisa tikus yang masih hidup pada hari ke 15 (T3) terhitung mulai dari awal perlakuan atau lima hari setelah induksi terakhir, kemudian tikus dikorbankan nyawanya dan dibuat pewarnaan imunohistokimia sel beta pankreas. Pengukuran kadar insulin dilakukan terhadap sampel darah yang telah diambil dan telah dipisahkan serumnya. Kemudian jaringan diinkubasi dalam antibodi sekunder yang terkonjugasi dengan biotin (Linker reagent biotinylated anti mouse and anti rabbit IgG) dan inkubasi pada suhu 37oC selama 20 menit. Untuk melihat ada tidaknya interaksi, data dianalisis dengan general linear model (GLM) dengan menggunakan software Minitab 16
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