HISTOMORPHOMETRY OF SPLEEN RED TILAPIA (OREOCHROMIS NILOTICUS) CULTIVATED WITH DIFFERENT AERATIONAND FILTRATION

Abstract

Cultivation of red tilapia (Oreochromis niloticus) requires good water quality. Physiological processes and organ structures can be disrupted when water quality including dissolved oxygen, water temperature, pH, salinity and ammonia exceeds the normal threshold. Aeration and filtration in fish farming can help improve water quality which affects the improvement of physiological processes and spleen function of red tilapia. The study was purposed to analyze the use of aerators, filters and a combination of both on the histomorphometry and structure of the spleen of red tilapia. This study used a completely randomized design with a 2×2 factorial arrangement. Each main factor consists of two levels, such as aeration i.e., single aeration and double aeration and filtration i.e., without filter and with filter. The experimental fish in the study were 24 red tilapia fish which were divided into four treatment groups, which included single aeration without filter, double aeration without filter, single aeration with filter, and double aeration with filter. The results showed that the interaction between aeration and filters had no significant effect (P>0.05) on spleen weight, Relative Spleen Weight (RSW), red pulp diameter, white pulp diameter, melanomacrophage center (MMC) diameter, and MMC cell diameter. The conclusion of this study was that the aeration, filters and combination of aeration and filters could maintain the chemical quality of water so there was no adverse effect on the histomorphometry and structure of the spleen of red tilapia