Abstract
Fibrillar matrices (viroplasms) have been described for a number of viruses, mainly from studies of thin-sectioned infected cells. We have recently noted some structured matrices (observed in negatively-stained preparations) that were associated with swine kidney (SK) cells infected with an Adenovirus, infectious canine hepatitis. These cellular fragments may bear some special relationship to the process of viral maturation.Virus was propagated in primary swine kidney cell monolayers. Cell culture harvests were pooled and clarified by centrifugation at 1000 X g for 20 min at 4 C. The pelleted materials were dispersed in 5 to 10 times their volume of distilled water and sampled directly for phosphotungstate (PTA) negative staining: one drop of the suspension was diluted with ca. 15 drops of 1% PTA which contained a trace of bovine serum albumin (Fr. V); the sample was then applied to carbon-coated collodion-filmed grids with a Vaponefrin all-glass nebulizer (Brenner & Horne. Biochim. Biophys. Acta 34: 103 (1959). The process of nebulizing appeared to rupture those cells that reached the specimen grid permitting examination of cellular fragments in great variety.
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