Abstract
A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to millimeters, but in bulk tissues the confounding effects of molecular absorption and strong multiple scattering of light often lead to a dramatic reduction in scatter contrast and specificity. It is demonstrated that the SLS using structured high spatial frequency illumination and detection to probe the tissue achieves direct, absorption-independent, high-resolution maps of the scattering response. The scattering response is observed to be dependent on both the wavelength and spatial frequency of choice, indicating a potential for multiscale probing of ultrastructural changes in superficial tissue layers. This methodology can be easily applied in most wide-field imaging systems.
Highlights
The elastic scattering of light offers a highly sensitive probing mechanism to characterize changes in morphology and ultrastructure at various size scales ranging from nanometers to millimeters.[1,2]
In conventional wide-field imaging of dense random media, such as biological tissue, the sensitivity of scattered light to ultrastructural changes is often reduced by the strong influences of multiple scattering events and rendered less specific because of the
It is shown that such selective high spatial frequency probing makes the tissue response insensitive to variations in absorption and concomitantly very sensitive to scattering changes
Summary
The elastic scattering of light offers a highly sensitive probing mechanism to characterize changes in morphology and ultrastructure at various size scales ranging from nanometers to millimeters.[1,2] Creative ways to measure light scattering signals can allow macroscopic measurements to inform the microscopic and even submicroscopic properties of a variety of materials as diverse as metal surfaces and cell suspensions. In contrast to the traditional SFDI approach, the SLS technique samples tissue responses only at high spatial frequencies (typically > 0.5 mm−1).
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