Abstract
We utilized these NIR QDs to demonstrate the utility of an image processing algorithm capable of determining the relative concentration ratios of two distinct emitters at various tissue depths. This algorithm is based on the dual probe imaging method, which we modified for use in wide-field imaging systems. The algorithm was validated using skin-mimicking tissue phantoms that were used to vary the effective imaging depth from 0-1.2 mm. The algorithm correctly determined concentration ratios of a two-color QD system for variations in concentration ≥ 10%, independently of depth. These contrast agents and the imaging approach are being adapted for use for in vivo targeted tumor imaging.ng adapted for use for in vivo targeted tumor imaging.
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