Abstract

Photosystem I particles from spinach were studied with linear-dichroic absorbance-detected magnetic resonance in zero-magnetic field. The microwave-induced triplet-minus-singlet (T−S) spectra and the linear-dichroic (LD) T−S spectra were recorded for the |D| + |E| and the |D| − |E| microwave transitions of the triplet state of the primary donor, 3P700. From these data the directions of the optical transition moments contributing to the T−S spectra in the 600−750 nm region, with respect to the triplet x- and y-axes of 3P700 were obtained. The orientation of the optical QY-transition moment of P700 relative to the triplet x- and y-axis is found to be 35 ± 2° and 56 ± 1°, respectively. A comparison is made with data obtained for monomeric chlorophyll (Chl) a in two glasses. The orientation of the QY-transition moment with respect to the in-plane triplet x- and y-axes of 3P700 differs from monomeric Chl a in the two glasses. This difference is ascribed to the different environments of P700 and Chl a, rather t...

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