Abstract
The triplet state of reaction centers of the HL(L173) and HL(M202) mutants of Rhodobacter sphaeroides, whose primary donor is a bacteriochlorophyll-bacteriopheophytin (BChl-BPh) heterodimer instead of a BChl-BChl homodimer, has been studied with absorbance-detected magnetic resonance in zero-magnetic field. The zero-field splitting parameters of the triplet state of the two heterodimers are close to those of monomeric 3BChl a, indicating that the triplet state is largely localized on the BChl-half of the heterodimers, but not identical, demonstrating that the two BChls of the native primary donor are inequivalent. The microwave-induced triplet-minus-singlet absorbance-difference spectra of the two mutant reaction centers differ in the Q Y-absorption region of the BChls that are located close to the dimer. The latter difference is attributed to differences in interaction between the dimer-BChls and the two adjacent BChls for the two mutants.
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