Abstract
Genomic DNA for Na,K-ATPase α 1 subunit was obtained from libraries of horse kidney genomic DNA in Charon 4A and in EMBL3 bacteriophages by screening with the full sized cDNA probe of the α 1 subunit of rat Na,K-ATPase as probe. The gene spans 30 kb and consists of 23 exons and 22 intervening sequences. Intron-exon boundaries were analyzed. The protein-coding nucleotide sequence encodes 1016 amino acids with an M r of 112 264. The putative amino acid sequence of horse α 1 is 96–97% homologous to those of other mammalian species.
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