Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L.

Abstract

An acidic polysaccharide, named as p-LBP, was isolated from Lycium barbarum L. by water extraction and purified by decoloration, ion exchange chromatography, dialysis and gel chromatography, successively. The primary structure analysis was determined by HPAEC-PAD, HPSEC, FT-IR, GC–MS, and NMR. The results showed p-LBP was a homogeneous heteropolysaccharide as a pectin molecule with an average molecular weight of 64kDa p-LBP was an approximately 87nm hollow sphere in 0.05mol/L sodium sulfate solution determined by HPSEC-MALLS, DLS and TEM. A discussion of degradation patterns gave the detailed structural information of p-LBP. Therefore, the results from degraded fragments elucidated that the backbone of p-LBP was formed by →4-α-GalpA-(1→, repeatedly. Partial region was connected by →4-α-GalpA-(1→ and →2-α-Rhap-(1→, alternatively. On the C-4 of partial →2-α-Rhap-(1→ residues existed branches forming by →4-β-Galp-(1→, →3-β-Galp-(1→ or →5-α-Araf-(1→, while on the C-6 of partial →3-β-Galp-(1→ residues existed secondary branches forming by terminal-α-Araf, terminal-β-Galp or →3-α-Araf-(1→.