Abstract
Objective: The objective of this study was to investigate the antimicrobial activity of Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis ATCC12228 and Propionibacterium acnes DMST 14916.Methods: Fungal fruiting bodies were extracted with 95% ethanol and ethyl acetate, and then, vaporized. The antimicrobial activities were determined by the disc diffusion method against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 skin infectious pathogens. A minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) for those crude extracts were determined. Finally, the chemical profile of crude extract was determined by using thin layer chromatography and GC-MS.Results: The result demonstrated that the ethanolic extraction had more active fractions with an MIC of 0.5 mg/ml against the growth of Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 and also showed a minimum inhibitory concentration (MBC) at a concentration of 1.0 mg/ml, while ethyl acetate-based solvents failed to develop on TLC according to Retention factor (Rf) values of 0.71-0.76. The GC-MS was applied to investigate the chemical profile of crude extract of Phellinus linteus, revealing a component of hexadecanoic acid and 9, 12-octadecadienoic acid.Conclusion: Phellinus linteus fruiting body extracts have great potential as antimicrobial compounds against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228. Thus, they can be used in the treatment of infectious diseases caused by bacterial pathogens.
Highlights
In recent years, the increasing antimicrobial resistance called superbugs has driven a critical need to develop a novel antimicrobial agent [1]
The antimicrobial activity results of Phellinus linteus fruiting body extracts are summarized by the paper-disc diffusion method as shown in table 1
The highest inhibitory activity was determined against S. epidermidis ATCC12228 and P. acnes DMST14916 in a clearing zone of 15.67±1.15 and 12.67±1.53 mm, whereas ethyl acetate extract showed no inhibition zone
Summary
The increasing antimicrobial resistance called superbugs has driven a critical need to develop a novel antimicrobial agent [1]. Several novel antibiotic agents that are most suitable for use in the hospital environment have been developed to combat both Gram-positive and Gram-negative bacteria, especially screening active biomolecules with effective pharmacologic properties and drug safety with a return to treatment using traditional medicines. Phellinus linteus has been found to produce several biologically active compounds that are usually associated with cell walls. These compounds have been suggested to contribute to the enhancement of immunity [6] and tumourretarding effect, antioxidant [7,8,9], anti-inflammatory [10] and antimicrobial activities, [11] and good therapeutic effects on diabetes and obesity [12]
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