Abstract
To explore bioactive polysaccharides from the byproducts of squid processing, a heteropolysaccharide, named SV2-1, was isolated from the viscera of squid Ommastrephes bartrami by autolysis, anion-exchange and gel-permeation chromatography and measured for its neuroprotective activity. It was a homogeneous polysaccharide with a molecular weight of 2.3 kDa by HPSEC analysis. SV2-1 contained glucuronic acid, galactosamine and fucose in the ratio of 1.0:1.1:1.2. Its structural characteristics were elucidated by methylation analysis, gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR). The backbone of SV2-1 was composed of alternant →4)-α-l-Fucp-(1→ and →3)-β-d-GlcUA-(1→ Most of →4)-α-l-Fucp-(1→ (90%) was substituted by single α-d-GlcNAc as the branches. SV2-1 can protect against the death of PC12 induced by 6-OHDA, and effectively improves cell viability and reduces extracellular LDH release in PC12 cells after injury. Moreover, SV2-1 significantly increases SOD activity but decreases MDA levels.
Highlights
Squid is considered to be important seafood, which is well known for its wide varieties, wide spreading distribution, abundance, and fishing production
Autolysis using the complex mixture of endogenous proteases under proper hydrolysis conditions can produce squid byproduct hydrolysates
These results indicated that SV2-1 displayed a protective effect against oxidative stress on PC12 cells [20]
Summary
Squid is considered to be important seafood, which is well known for its wide varieties, wide spreading distribution, abundance, and fishing production. It is representative of the increased waste production in the seafood industry [1,2]. Proteolysis is en efficient way to produce bioactive peptides and free amino acids from the discarded byproducts with high nutritive value and functional properties, which can be used in the food and medicine industries [3]. Peptides with angiotensin I-converting enzyme inhibitors activity were produced from the autolysates of squid liver and mantle muscle [4]. Squid viscera autolysates showed strong in vitro antioxidative activities [5]
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