Abstract
Lipid vesicles are widely used as models for cell membranes and extracellular vesicles, hosts for membrane protein studies, and containers for hydrophilic biotherapeutic molecules. Vesicle solutions are usually prepared at a specific lipid concentration; however, because vesicles are solvent-filled structures, the corresponding volume fraction of vesicles is at least a factor or 3 times higher than the corresponding lipid volume fraction and critically depends on the vesicle radii. Seemingly low lipid concentrations correspond to significantly higher vesicle volume fractions and closer face-to-face distances between their surfaces than may be expected.
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