Measurement of mechanical properties of naked cell membranes using atomic force microscope puncture test

Abstract

Cell membrane defines the physical boundary of the cell, maintains their shape and volume, and meanwhile dominates the cells response to mechanical force. There are many limitations in the mechanical analysis of naked cell membrane, such as cell condition, the supporting of cytoskeleton and cytoplasm. To reduce these influences, non-supported membrane was prepared on high-ordered pore array silicon substrate. Rupture forces and Young's modulus of three non-supported membranes, red blood cell membrane, somatic cell (MDCK) membrane and phospholipids membrane, are obtained quantitatively using AFM puncture tests. Results indicate that the sequence of both rupture forces and Young's modulus of the three membranes is MDCK cell membrane > red blood cell membrane > phospholipids membrane. The determinant of naked cell membrane's mechanical properties is the constituent of membrane itself, including the quantity and distribution of membrane proteins. Focused on the distribution of membrane proteins, two proposed models of cell membrane—the semi-mosaic model of red blood cell membrane and the Protein Layer-Lipid-Protein Island (PLLPI) model of nucleated mammalian cell membrane can be used to explain the different mechanical properties of naked cell membranes.