Abstract
Publisher Summary This chapter discusses the isolation, and the physicochemical characteristics of immunoglobulin E (IgE) properties of antigenically and biologically active structural regions of IgE, levels of IgE in disease, and detection of antibody activity in the IgE Class. IgE represents a minor, but a distinct class of proteins present in the serum of man and higher primates, and possibly also in the serum of other species. The detection and quantitation of IgE require very sensitive methods. Immunoglobulin E is elevated 4-30 times normally in various diseases, among which atopic disorders and parasitic infestations appear to be the most prominent. Pathological amounts of IgE have also been found in the serum of patients with γE myeloma. The best way to determine IgE is to use the immunochemical methods based upon the reaction between IgE and the antibodies specific for the class-related antigenic determinants of IgE. Gel diffusion methods, such as single radial diffusion in gel are handicapped by low sensitivity; radioimmune assays have proven to be superior.
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