Abstract
A compound of {[Ni(5,5´-Dimethyl-2,2´-bipyridine)3](SCN)2}2(a) was synthesized and characterized by FT-IR, UV–Vis spectroscopy, elemental analysis, luminescence and X-ray crystallography. The single crystals were obtained by slow crystallization from a methanol solution. The complex is composed of nickel cation chelated by three neutral bipyridine ligands and two (SCN−) ions outside the coordination sphere. The nano form of the synthesized complex (b) was prepared by a sonochemical process and confirmed with XRD, SEM and FT-IR. The average size of the particles was 37 nm from SEM.Thermodynamic parameters (∆H°, ΔS° and ΔG°) calculated from FS-DNA interaction of complexes showed that hydrogen bonding and van der Waals interactions have an essential function in the interaction of DNA–Ni(II) complex, and the interaction mode is groove binding. Viscosity measurement illustrated that relative viscosity of DNA remained unchanged with the adding concentrations of complexes. Circular dichroism (CD) spectra showed that the structure of DNA was changed.The antibacterial properties were investigated in vitro against standard Gram-positive and Gram-negative bacterial strains. The results of antibacterial tests showed that (a) was a stronger antibacterial agent than the free ligand, and that the antibacterial effect of (b) was stronger than the one of (a). The cytotoxicity activity experiments against MCF-7, KB and A549 cells revealed low to moderate antiproliferative activity of the complex (b) against cancer cells. The molecular docking results exhibited groove mode of binding, confirming previously obtained data from spectroscopy, viscometry and CD.
Published Version
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