Abstract
Membrane protein often needs lipid bilayer membrane to stabilize its structure and to fulfill its functions. However, the presence of lipid bilayer membrane makes the structural study on the protein very difficult. Here we proposed to use X-ray scattering contrast variation method in which membrane protein (with lipid membrane) samples will be measured in a series of solutions with various sucrose concentration. X-ray scattering intensity measures the scattering length/capability difference between sample/solute and buffer solution/solvent. Since X-ray scattering lengths of protein and lipid are different and scattering length of sucrose buffer changes along with its concentration, the ratio of X-ray scattering contribution from protein part and lipid part varies along with the sucrose concentration. In such way, we can separate the scattering contribution of protein part from that of lipid. We also simultaneously fit the data sets collected from the sucrose concentration series measurements and obtain the 3-D low resolution structure for the protein and the lipid at the same time. Comparing to neutron scattering contrast variation method, this X-ray contrast method is less expensive and can provide wider q range due to the availability of high flux X-ray sources. A few membrane protein examples will be presented. Figure. SAXS data for membrane protein in various sucrose solution. In this presentation, I will also discuss the capabilities of beamline 12-ID-B of the Advanced Photon Source at Argonne National Laboratory. Acknowledgement: Use of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory, was supportedby the U.S. DOE under Contract No. DE-AC02-06CH11357.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call