Abstract
The structure of the neutral sugar side-chains of pectic substances extracted with water (WSP), dilute HCl (HP), and endopectinlyase (AIR/PEL) from an alcohol-insoluble residue (AIR) from the pulp of grape berries was investigated by using chemical methods (methylation, carboxyl-reduction, and periodate oxidation), enzymic degradation with an α- l-arabinofuranosidase, and 13C-n.m.r. spectroscopy. The side chains of WSP are essentially a type II arabinogalactan, whereas those of HP and AIR/PEL are arabinan-like structures associated with minor proportions of type I and II arabinogalactans. The side chains are ∼80% degraded by the α- l-arabinofuranosidase, indicating that the α- l-arabinofuranosyl residues are mainly 3-linked to the (1→6)-galactan side-chains of the type II arabinogalactan. The three pectic fractions contain minor amounts of proteins rich in hydroxyproline and serine.
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