Abstract
The structural order of cholesteryl acetate-dimyristoylphosphatidylcholine (DMPC)-water mixtures was examined at room temperatures using Raman spectra in the CH and CC stretch regions. Intensity ratios I 2890/I 2850, I 2830/I 2930 and I 1064/I 1089 were used as structural parameters to measure the order of DMPC acyl chains. These three ratios pass through a well defined minimum at ca. 14 mol% cholestryl acetate. These data also show that cholesteryl acetate disrupts the packing order of the DMPC acyl chains from 0 to ca. 35 mol% cholesteryl acetate. As the concentration of cholesteryl acetate is increased from ca. 14 to ca. 35 mol% the DMPC acyl chains become more ordered and cholesteryl acetate appears to form a crystalline phase that remains inn suspension with the lecithin. As more cholesteryl acetate is added, these crystalline regions become larger and begin to form a precipitate which is structurally identical to those particles which remain in suspension.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call