Structural requirements for species-specific induction of the sperm acrosome reaction by sea urchin egg sulfated fucan

Abstract

The sulfated fucan (SF) of egg jelly induces the acrosome reaction (AR) of sea urchin sperm. Strongylocentrotus franciscanus ( Sf) SF is sulfated only at the 2-position. Strongylocentrotus purpuratus ( Sp) has two SF isotypes, each one being female specific. One is rich in sulfate at both the 2- and 4-positionS (SF-1), and the other is rich in sulfate at the 4-position, but not the 2-position (SF-2). Sf SF is poor at inducing the AR of Sp sperm, presumably due to lack of 4-sulfation. Sp SF-1 is better at inducing the AR of Sf sperm than Sp SF-2, hypothetically due to increased 2-sulfation. Chemical oversulfation of Sf SF increases the percentage of AR of Sp sperm, showing that 4-sulfation is important for recognition of SF by Sp sperm. Chemically oversulfated Sp SF-2 is better at inducing the Sf sperm AR, presumably because of increased 2-sulfation. The species, Strongylocentrotus drobachiensis ( Sd), has an SF-2 that is exclusively 2-sulfated (like Sf), except the glycosidic linkage in Sd is α(1→4), whereas in Sf it is α(1→3). Sd SF-2 does not induce the AR of Sf sperm, showing the strict requirement for the α(1→3) linkage in recognition between Sf sperm and SF. Egg jelly from Echinometra lucunter ( El) contains sulfated galactan (SG) which differs from Sf SF only in that the monosaccharide is l-galactose, not l-fucose. This SG and Sf SF are equally potent in inducing the AR of Sf sperm, showing that modification at C6 of l-fucose is not important for proper recognition between SF and Sf sperm receptors. This system permits study of the structural basis for recognition between sulfated polysaccharide and receptors controlling signal transduction pathways in animal cells.