Structural Preservation and Analysis of Biological Macromolecules in Cryogenic Microscopy for Future Drug Discovery

Abstract

Lately, cryogenic electron microscopy (cryo-EM) has emerged as a cutting-edge biophysical technique for structural analysis of biological macromolecules and assemblies. In structural analysis, structural preservation of samples such as isolated cell organelles, viruses, bacteria, eukaryotic cells and other samples for soft-matter applications is extremely important. Due to the advantage of cryo-EM technique to preserve the structures of biological macromolecules, there is a tremendous research interest in Cryo-EM among biotechnologists, medical professionals and biomaterials scientists that seek an unaltered view on the architecture of a vast array of clinically important biological specimens. In this perspective, we have described a brief overview of the recent progress in this field.