Structural, optical and antimicrobial studies of 3β-acetoxycholest-5-ene, 3β-acetoxy-6-nitrocholest-5-ene and newly synthesized steroidal pyrazolones

Abstract

A convenient synthesis of a new series of nano steroidal pyrazolones is reported. They were characterized by X-ray diffraction, scanning electron microscopy, UV–vis light, Fourier transform-infrared spectroscopy, 1H and 13C nuclear magnetic resonance, mass spectroscopy and analytical data. The X-ray diffraction patterns at room temperature showed that two nano steroids, 7 and 8, are formed in single phase with hexagonal crystal symmetry, while nano steroid 9 is formed with orthorhombic crystal symmetry. Compounds 1–3 are formed with hexagonal, monoclinic and tetragonal crystal symmetry, respectively. Scanning electron microscopy showed that the crystals of nano steroids 7–9 are brick-shaped agglomerates with less sharp edges and a rough surface with a few microcrystals, while the homogeneous crystal of nano steroid 3 has an approximate spherical morphology of nano particles conjoined to the chains. UV–vis absorption analysis showed that the band gap energy of nano steroids 7 and 8 was 3.70eV and 4.61eV, while that of 2 and 3 was 4.38eV and 4.27eV, respectively. Nano steroids 2, 3 and 7–9 were screened for antimicrobial activity against various strains; nano compound 7 showed potential antimicrobial activity.