Structural identification through GC mass spectrophotometer and determine anti lithiotic activity of hibiscus rosa sinensis by using ethylene glycol induced method

Abstract

The present study investigated the antiurolithiatic effect of standardized ethanolic extract of Hibiscus rosa sinensis by structural elucidation of the responsible compound for the activity as well as Ethylene glycol model induce calcium oxalate crystal in Albino rats. The chemical structural elucidation of H. rosa sinensis performed by phytochemical study and GC-Mass spectrophotometry. Calcium oxalate urolithiasis was induced in rats in rats by ethylene glycol -ammonium chloride feeding in drinking water. Antiurolithiatic activity of H. rosa sinensis was evaluated in 2 different doses (300,600 mg/kg) in curative and preventive regimen by estimating the histological changes in kidney and biochemical change in urine, serum. Sodium oxalate was used as reference standard drug. Ethylene glycol-ammonium chloride feeding caused an increase in urinary volume, oxalate, total protein, phosphate and uric acid levels along with a decrease in urinary excretion of calcium, magnesium and citrate. Test group -1 and Test group-2 animals treated with H. rosa sinensis decrease the amount of calcium, oxalate, citrate, which is responsible for induce calcium oxalate crystals in significant amount. Histological study revealed minimum damage and a smaller number of calcium oxalate deposits in the kidney of ethylene glycol treated rats. These results indicated that the hibiscus rosa sinensis reduced and prevented the growth of urinary stones. However, the sodium citrate is more effective than the H rosa sinensis (600 mg/kg more effective than hibiscus 300 mg/kg) in the treatment of urolithiasis. Keywords: Hibiscus Rosa Sinensis, Kidney Stone, Sodium Citrate, Ethylene Glycol, Calcium Oxalate.