Abstract
The structural organization of the biological material in the nucleoli (Nucleolus-Like Bodies (NLBs)) in mouse GV oocytes has been studied with atomic force microscopy (AFM), scanning electron microscopy (SEM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Experimental samples are prepared by quick plunge freezing, followed by freeze-drying, embedding in Epon epoxy resin, and sectioning with a microtome. The biological material in the NLBs is found to be organized as a domain structure of densely packed granules with sizes from tenths of a micron to a micron.
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