Abstract
The structure of the antigenic O-polysaccharide (O-PS) of the lipopolysaccharide (LPS) produced by the enterohemorrhagic strain of Escherichia coli O48:H21 (EHEC) has been elucidated. The O-PS obtained by mild acid hydrolysis of the LPS had [ α] D +95 (water) and was composed of l-rhamnose ( l-Rha), d-galactose ( d-Gal), 2-amino-2-deoxy- d-glucose ( d-GlcN), 2-amino-2-deoxy- d-galactose ( d-GalN), and d-galacturonic acid ( d-GalA) (1:1:1:1:1). From the results of methylation analysis, mass spectrometry, 2D NMR, and DOC-PAGE, the O-PS was shown to be a high molecular mass polymer of a repeating pentasaccharide unit having the structure: [Display omitted] The d-Gal pA non-reducing end groups in the O-PS were partially O-acetylated (∼30%) at the O-2 and O-3 positions and the degree of acetylation was variable from batch to batch cell production.
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