Abstract
In recent years, the biological activity of plant polysaccharides has received increasing attention. Polysaccharides, as one of the main components of lilies, have pharmacological effects in regulating immunity, anti-tumor, antioxidant, antibacterial, and hypoglycemic effects. To systematically analyze the structural characteristics of the polysaccharide, the polysaccharide LP-1 was prepared from Lilium lancifolium bulbs by water extraction and ethanol precipitation, ion exchange chromatography and gel filtration chromatography. Structural characterizations show that the weight-average relative molecular weight of LP-1 is 5.3 kDa. LP-1 consists of mannose and glucose at a molar ratio of 1.4:1. Its primary structure is Glcp-α-D-(1 → {4)-β-D-Glcp-(1 → 4)-β-D-2-O-acetyl-Manp-(1 → [4)-β-D-Glcp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Manp-(1 → 4)-β-D-Manp-(1 → 4)-β-D-Manp-(1 → 4)-β-D-Manp-(1]2}2 → 4)-α-D-Glcp. Acetylation occurs at the O-2 site of mannose. Acetyl substitution degree is 8.21%. The results of structure elucidation showed that LP-1 was a low-molecular weight linear O-acetylated glucomannan. The results of oxidative stress experiments indicate that LP-1 exerts antioxidant effects on HUVEC by activating the Nrf2/HO-1 signaling pathway, thereby improving endothelial cell damage induced by H2O2, and has the potential to be developed as an antioxidant drug.Graphical
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