Abstract
We have investigated the morphology of dimyristoyl phosphatidyl choline (DMPC)—cholesterol mixed monolayer formed on the water surface by dropping method using surface tension measurement (STm), Brewster angle microscopy (BAM), and infrared external reflection spectroscopy (IERS). STm results showed negative deviation of the limiting molecular area (A0) of cholesterol occurred when cholesterol was added to the DMPC monolayer. BAM images showed the expandable DMPC monolayer changed to the condensed rigid monolayer at more than cholesterol mole fraction (xChol) 0.4. IERS recordings showed that the addition of cholesterol at xChol = 0.4 occurred structural change from gauche- to trans- conformation of two DMPC molecule alkyl chains. From these results, it is found that cholesterol molecule has specific properties that cause structural transition of DMPC molecule alkyl chains.
Highlights
We have investigated the morphology of dimyristoyl phosphatidyl choline (DMPC)—cholesterol mixed monolayer formed on the water surface by dropping method using surface tension measurement (STm), Brewster angle microscopy (BAM), and infrared external reflection spectroscopy (IERS)
We have reported the cholesterol-mixed effect on the morphology of expandable dimyristoyl phosphatidylcholine (DMPC) (Figure 1(b)) monolayer formed on the water surface for various cholesterol mole fractions by dropping method
We have investigated the cholesterol addition effect on the DMPC monolayer formed by dropping method
Summary
(surfactant) are two-dimensionally arranged to a monomolecular layer at the air-water interface. The techniques of thermal analysis, ATR-FTIR and NMR spectroscopy, and molecular simulation [5] [12] [13] [14] [15] [16] showed that the structure of DMPC molecule exchanged from liquid-disordered (Ld) phase to liquid-ordered (Lo) phase with an increase in cholesterol mole fraction. In this communication, we have reported the cholesterol-mixed effect on the morphology of expandable dimyristoyl phosphatidylcholine (DMPC) (Figure 1(b)) monolayer formed on the water surface for various cholesterol mole fractions by dropping method. The results have been interpreted to work out an addition effect of cholesterol in the structural change of DMPC molecules
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