Abstract
Dendrobium nobile Lindl., an epiphytic herb distributed in the Southeast Asia, is used as a tonic and antipyretic herbal medicine in China. In this study, a water-soluble acidic heteropolysaccharide, DNP-W4, containing mannose, glucose, galactose, xylose, rhamnose, and galacturonic acid, in the molar ratios of 1.0:4.9:2.5:0.5:1.0:0.9, was obtained from the stems of Dendrobium nobile Lindl. Using methylation analysis, partial acid hydrolysis, pectolyase treatment, NMR, and ESI-MS, the structure of DNP-W4 was elucidated. The obtained data indicated that DNP-W4 was a complex heteropolysaccharide and possessed a backbone composed of (1→4)-linked β-d-Glcp, (1→6)-linked β-d-Glcp, and (1→6)-linked β-d-Galp, with substitutes at O-4/6 of Glcp residues and O-3 of Galp. The branches of DNP-W4 were composed of terminal Manp, (1→6)-linked β-d-Manp, (1→3)-linked β-d-Glcp, β-d-Glcp, β-d-Galp, (1→4)-linked α-d-GalAp, (1→2)-linked α-L-Rhap, and Xylp. DNP-W4 had little immunological activities, but its derivatives had immuno-stimulating activities to some extent.
Highlights
IntroductionPolysaccharides from medicinal plants have attracted great attention in the food industry owing to their wide variety of bioactivities, especially antioxidant and immuno-stimulating activities, indicating that polysaccharides from medicinal plants can offer a safe and natural source of antioxidants and immune-stimulants for application in the food industry [1,2,3]
In the recent years, polysaccharides from medicinal plants have attracted great attention in the food industry owing to their wide variety of bioactivities, especially antioxidant and immuno-stimulating activities, indicating that polysaccharides from medicinal plants can offer a safe and natural source of antioxidants and immune-stimulants for application in the food industry [1,2,3].They may be added to foods as additives or consumed directly as functional foods
In the immunological activity assay (Table 3), DNP-W4 had little activities even at the at 50 μg/mL and 100 μg/mL dose on T and B lymphocytes. These results suggested that the side chains of DNP-W4 had effect on the expression of immunological activity
Summary
Polysaccharides from medicinal plants have attracted great attention in the food industry owing to their wide variety of bioactivities, especially antioxidant and immuno-stimulating activities, indicating that polysaccharides from medicinal plants can offer a safe and natural source of antioxidants and immune-stimulants for application in the food industry [1,2,3]. They may be added to foods as additives or consumed directly as functional foods.
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