Abstract
Abstract Dendrobium nobile is commonly used as a Yin tonic for reinforcing body fluid and nourishing blood in Southeast Asia. In this paper, a pectic polysaccharide DNP-W5 was obtained from the stems of D. nobile . It contained mannose, glucose, galactose, xylose, rhamnose and galacturonic acid in molar ratios of 3.1:8.1:8.2:0.6:4.2:3.9. About 21% of the galacturonic acid existed as methyl ester and O -acetyl groups were approximately 6.9%. The structural analysis indicated that DNP-W5 possessed a backbone of a disaccharide of [→4)-α-GalA p -(1 → 2)-α-Rha p -(1→] with branches at O -4 of the Rha p and O -3 of the Gal p A. The side chains consisted of galactosyl, mannosyl, glucosyl, and xylosyl residues. In vitro bioactive tests, DNP-W5 displayed remarkable immunoenhancing activities on T- and B-lymphocytes and its branches and acetyl groups are very important for the expression of the immunological activity.
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