Abstract
The present study was performed to find out the structural similarity of hydroalcoholic extract of abutilon indicum and standard non-steroidal anti-inflammatory drugs (NSAIDs) using various analytical and chromatography techniques. The characterization was performed by fourier transform infrared spectrophotometer (FTIR), UV–Vis spectrophotometer, thin layer chromatography (TLC), and reverse-phase high phase liquid chromatography (RP-HPLC). Paracetamol and aceclofenac were used as standard NSAIDs. The FTIR peaks obtained for abutilon indicum were at wavenumber 2933.81, 2359.61, 1459.90, 762.73, 1640.09, 1559.74, and 3253.75 cm−1 confirms the presence of aromatic acid, hydroxyl group, and carboxylic groups. In addition, the UV–Vis profile showed peaks at 274 nm and 244.5 nm for abutilon indicum in methanol and sodium hydroxide respectively. Further, the TLC method showed the retention factor (Rf) values at 0.38 and 0.36 for abutilon indicum which were almost similar to Rf values of paracetamol and aceclofenac. The peak and retention time from RP-HPLC for abutilon indicum showed the values at 3.640 and 2.603 that were in accordance with standard NSAIDs. Thus, this study can give an insight that abutilon indicum does contain structures similar to paracetamol and aceclofenac which are responsible for their analgesic activity.
Published Version
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