Abstract
Objectives: To explore the polarizing color profile in connective tissue stroma of Picro-Sirius Red (PSR) stained sections of benign and malignant salivary gland tumors (SGT) and to assess the possible utility of this method as a diagnostic tool. Materials and Methods: A total number of 60 Paraffin blocks of benign (30 cases) and malignant (30 cases) salivary gland tumors were selected from the archives of our institute and regional cancer hospital. 30 cases of Mucous Extravasation Phenomenon (MEP) were selected as control. Salivary gland tumors and control were stained with PSR stain and examined under polarizing microscope. We counted 50 thin and 50 thick collagen fibers in each section of benign and malignant SGT and were categorized as Greenish Yellow (GY) or Yellowish Orange (YO). Statistical analysis was performed by using oneway ANOVA and Paired t-test. Results: We found similar presentation of polarizing color arrangement for thin collagen fibers (80-85% GY and 15-20% YO) in both benign and malignant SGT and MEP. In malignant SGT, thick collagen fibers exhibited different polarizing color (80% GY) than benign SGT and MEP (12-14% GY). Polarizing color profile of collagen in malignant SGT displayed a different presentation as compared to benign SGT and control (MEP) and differences were statistically significant(p<0.05). Conclusion: This study concluded about the difference of stromal collagen fibers in benign and malignant SGT. Malignant SGT suggesting loosely arranged collagen fibers could be involved in further growth and invasion of disease along with other cellular and molecular events. Keywords: Collagen, Mucous Extravasation Phenomenon, Picro-Sirius Red (PSR) Stain, Polarized Microscopy, Salivary Gland Tumor
Highlights
Salivary gland tumors characterize the most complicated and diverse cluster of tumors seen in the head and neck
The present study revealed that there was a significant difference in polarizing color profile of malignant and benign salivary gland tumors (SGT)
The polarization colors profile for thick collagen fibers, demonstrated an altered pattern in the malignant tumors, Adenoid Cystic Carcinoma (ADCC) and Muco-Epidermoid Carcinoma (MEC), as compared to the benign tumor, Pleomorphic Adenoma (PA) and Warthin’s Tumors (WT)
Summary
Salivary gland tumors characterize the most complicated and diverse cluster of tumors seen in the head and neck. Salivary gland tumors often presents a wide spectrum of tumor histomorphology, the neoplastic lesions are too numerous, histologically most heterogeneous and present a diverse clinical behavior. The histomorphogenesis of salivary gland tumors are complex and poorly understood[1]. The most common benign SGT are Pleomorphic Adenoma (PA) and Warthin’s Tumors (WT), comprising 75-90% of all salivary gland tumors. Most common malignant SGT are Adenoid Cystic Carcinoma (ADCC) (30%) and Muco-Epidermoid Carcinoma (MEC) (70%)[2]. Numerous theories are suggested and described in literature about histogenesis of SGT.
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