STRESS-PROTECTIVE EFFECTS OF NEW MEDICINAL FORM WITH GLYCINE AND MAGNESIUM CITRATE

Abstract

Introduction. Stress disorders are common in clinical practice, and their treatment requires selective medication choice. Among the drugs having anxiolytic and sedative effects, glycine and magnesium compounds are being intensively investigated and the options of producing sublingual tablets based on these substances seem to be promising. The aim of this study is to investigate the effects of glycine and magnesium citrate and their combination on glycine and magnesium citrate on somatic parameters (Selye's triad) under the stress conditions and to determine the most effective composition of the compounds.
 Materials and methods. The experiments were carried out on 76 white rats. The substances were administered sublingually and then the animals were kept in the small cages for 6 hours in order to simulate immobilization stress. At the end of the stress exposure, the rats were euthanized and the relative masses of the thymus and adrenal glands as well as a presence of ulcers and haemorrhages in the stomach were determined. Statistical processing was performed using the Statistica for Windows 8.0 software.
 Results and discussion. Immobilization stress resulted in a decrease in the relative weight of the thymus in the rats without significant changes in the weight of the adrenal glands and intense ulceration in the stomach. Prophylactic use of the compounds under the study and their combinations prevented the stress-induced involution of the thymus. The most pronounced protective effect was demonstrated by the combination of 100 mg glycine and 250 mg magnesium citrate and glycine itself. At the same time, the relative mass of the adrenal glands did not change significantly. The lowest incidence of ulcerative lesions was observed in the groups of animals who received the combinations of glycine (100 mg) and magnesium citrate (250-350 mg) or glycine (100 mg) separately. This scheme leads to a decrease in the number and area of gastric ulcers as compared to the control group. Magnesium citrate in doses of 250 mg and 350 mg was found not as effective in protecting the gastric mucosa.
 Conclusion. Acute immobilization stress causes involution of the thymus and gastric ulceration, but does not result in adrenal hypertrophy. According to the findings obtained by the Selye triad study, a combination of 100 mg glycine and 250 mg magnesium citrate and glycine separately in a dose of 100 mg have showed the highest prophylactic effect.