Abstract
A single subcutaneous insertion will stimulate the secretion of corticosterone. Rats pretreated by repeated daily subcutaneous needle insertions can be subsequently injected without the injection itself causing an increase in plasma corticosterone. This conditioning procedure will allow the study of the effect of parenterally administered materials in animals without the injection per se imposing an acute release of adenohypophyseal ACTH and of adrenal corticosteroids.
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