Abstract

Real-life stressors, such as university examination, cause an increase in sympathetic activity of the nervous system innervating the heart, and thus an increase in heart rate (HR). Our study aimed to detect changes in heart rate variability (HRV) during different stages of an exam in a group of 90 healthy university students (30 males and 60 females), over 4 h of monitoring divided into 1 h before, 2 h during, and 1 h after the examination. HRV was significantly highest after the exam, indicating release from stress, as compared to before and during the examination when stress was observable. Undergraduate students in different academic years did not differ in terms of stress, indicating the absence of adaptation to exam procedures. However, HR and R-R interval after the exam showed significant difference between first year undergraduate studies and first year of a graduate program, indicating a higher degree of confidence in graduate students. Results also suggest that HRV in females is significantly lower than that in males before and after examination, despite men having greater sympathetic input. In conclusion, the results of our novel study assessing stress in real-time examination show important gender differences, and lack of adaptation with academic study year.

Highlights

  • Heart rate variability (HRV) is a promising tool for assessing cardiovascular health and the degree of severity of cardiovascular diseases [1]

  • Students at the Lebanese University generally pass through a stressful period during the examination time; the main goal of this research was to study the changes in heart rate (HR) and heart rate variability (HRV) parameters, which reflect stress levels among university students just before, during, and after the exam takes place; subsequently, another aim was to assess such HRV changes based on gender

  • After the exam, SDNN significantly increased compared to before and during the exam (89.78 ms, P < 0.001), RMSSD significantly increased compared to the 2 hour period during the exam (46.55 ms, P = 0.05), and pNN50 was found to be significantly increased compared to before the exam based on post-hoc analysis (13.01%, P = 0.046)

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Summary

Introduction

Heart rate variability (HRV) is a promising tool for assessing cardiovascular health and the degree of severity of cardiovascular diseases [1]. HRV is affected by various factors including physical activity, such as running or practicing yoga, where subjects who exercise regularly possess lower HR and present an increase in HRV [3]; exercise has shown to slightly reduce the sympatho-vagal activity, inferred through the low frequency (LF)/high frequency (HF) ratio component of HRV [4]. This represents some kind of adaptation of the heart to external stimulators and its response to them. Other lifestyle factors, such as smoking and caffeine intake, may affect HRV when studying the heart response to a certain stressor

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