Abstract
During a screening for novel and useful actinobacteria in desert animal, a new actinomycete was isolated and designated strain TRM63209T. The strain was isolated from in vivo of a Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans ATCC 18,804. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM63209T were observed to be oval-shaped, with a smooth surface. Strain TRM63209T was found to grow optimally at 28 °C, pH 8 and in the presence of 1% (w/v) NaCl. The whole-cell sugars of strain TRM63209T were rhamnose ribose, xylose, mannose, galactose and glucose, and the principal polarlipids were found to be diphosphatidylglycerol, phos-phatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, phosphatidylinositol and an unknown phospholipid(L). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H6) (14.64%), MK-9(H2) (19.65%), MK-9(H8) (22.34%), MK-10(H2) (25.37%). The major cellular fatty acids were identified as iso-C16:0, 16:0, anteiso-C15:0, anteiso-C17:0, iso-C15:0 and Sum in Feature 3. Analysis of the 16S rRNA sequence showed that strain TRM63209T exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781T 98.20%. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and phylogenomic analysis also illustrated that strain TRM63209T should be assigned to the genus Streptomyces. The DNA G + C content of the strain was determined to be 70.2mol%. Average nucleotide identity (ANI) between strain TRM63209T and S. bungoensis DSM 41781T, Streptomyces phyllanthi PA1-07T, Streptomyces longwoodensis DSM 41677T and Streptomyces caeruleatus NRRL B-24802T were 82.76%, 82.54%, 82.65%, 84.02%, respectively. Digtal DNA-DNA (dDDH) hybridization were 26.30%, 25.10%, 26.20%, 29.50%, respectively. Therefore, it is concluded that strain TRM63209T represents a novel species of the genus Streptomyces, for which the name Streptomyces blattelae is proposed. The type strain is TRM63209T (CCTCC AA 2018093T = LMG 31,403 = TRM63209T).
Highlights
The genus Streptomyces was initially described in 1943 (Waksman and Henrici 1943) and more than 850 species with valid names of Streptomyces have been published (Zhang et al 2017)
Analysis of the 16S rRNA sequence showed that strain TRM63209T exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781T 98.20%
A multi-locus sequence analysis of five house-keeping genes illustrated that strain TRM63209T should be assigned to the genus Streptomyces
Summary
The genus Streptomyces was initially described in 1943 (Waksman and Henrici 1943) and more than 850 species with valid names of Streptomyces have been published (https://www.bacte rio.net/streptomyces.html) (Zhang et al 2017). Streptomyces are highly versatile and produce an abundant array of bioactive secondary metabolites (Genilloud 2017) that have been used as antibiotic, anti-carcinogenic, anti-helminthic and antifungal compounds. They are very important for biotechnology, medicine and agriculture (Barka et al 2015). Strain TRM63209T isolated from in vivo of an Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China(40°55’N,81°29’E). This strain inhibits the formation biofilm of Candida albicans ATCC 18804. On the basis of phylogenetic, phenotypic and genetic data, TRM63209T considered to a novel species of the genus Streptomyces, for which the name Streptomyces blattellae is proposed
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