Streptomyces albicerus sp. nov., a novel actinomycete isolated from the sediments of the Tailan River in Xinjiang, China.

Abstract

A novel actinomycete, designated TRM68295T, was isolated from the sediments of the Tailan River, Xinjiang, northwest China. The study of the polyphasic approach showed that the characteristics of the strain were consistent with the genus Streptomyces. Phylogenetic analysis indicated that the strain had a high sequence similarity with Streptomyces phaeochromogenes ATCC 3338T (97.9%). The diagnostic diamino acid of cell walls was identified as LL-diaminopimelic acid. The whole cell sugars were identified as ribose, xylose, glucose and galactose. The major fatty acids were iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C16:1ω9c, C16:0 and anteiso-C17:0. The major menaquinones were MK-9 (H10), MK-9 (H6) and MK-9 (H2). The polar lipids were composed of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylinositol mannoside (PIM). The G+C content in the draft genome sequence of the strain was identified as 70.0mol%. The average nucleotide identity (ANI) between strains TRM68295T and S. phaeochromogenes ATCC 3338T was 88.1%. Digital DNA-DNA hybridization (dDDH) value between strain TRM68295T and S. phaeochromogenes ATCC 3338T was 40.7%. The multilocus sequence analysis of five house-keeping genes (atpD, gyrB, recA, rpoB, and trpB) indicated that the MLSA distances between the strain and similar species were greater than the threshold of 0.007. Based on the above studies, strain TRM68295T is considered as a novel species of the genus Streptomyces, for which the name Streptomyces albicerus sp. nov. is proposed. The type strain is TRM68295T (= CCTCC AA 2018085T = KCTC 49272T).