Streptococcus mutans in atherosclerotic plaque: Molecular and immunohistochemical evaluations.

Abstract

To verify the presence of Streptococcus mutans (S.mutans) in atherosclerotic plaque (AP) using techniques with different sensitivities, correlating with histological changes in plaque and immunoexpression of inflammatory markers. Thirteen AP samples were subjected to real-time polymerase chain reaction (qRT-PCR), histopathological analyses, histochemical analysis by Giemsa staining (GS), and immunohistochemical analysis for S.mutans, IL-1β, and TNF-α (streptavidin-biotin-peroxidase method). Ten necropsy samples of healthy vessels were used as controls. All AP samples showed histopathological characteristics of severe atherosclerosis and were positive for S.mutans (100.0%) in qRT-PCR and immunohistochemical analyses. GS showed that Streptococcus sp. colonized the lipid-rich core regions and fibrous tissue, while the control group was negative for Streptococcus sp. IL-1β and TNF-α were expressed in 100% and 92.3% of the AP tested, respectively. The control samples were positive for S.mutans in qRT-PCR analysis, but negative for S.mutans, IL-1β, and TNF-α in immunohistochemical analyses. The detection of S.mutans in AP and the visualization of Streptococcus sp. suggested a possible association between S.mutans and atherosclerosis. The results obtained from the control samples suggested the presence of DNA fragments or innocuous bacteria that were not associated with tissue alteration. However, future studies are necessary to provide more information.