Streptococcus Colonial Growth of Dental Plaque Inhibition Using Flavonoid Extract of Ants Nest (Myrmecodia pendans): An in Vitro Study

Muhammad Harun Achmad,Sri Ramadhany,Filipus Eric Suryajaya

doi:10.4034/pboci.2019.191.19

Muhammad Harun Achmad, Sri Ramadhany + Show 1 more

Open Access

https://doi.org/10.4034/pboci.2019.191.19

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Abstract

Objective: To know the activity of resistance of flavonoid content in ant nest plant in decreasing the number of colonies S. mutans oral cavity of children as a medic herbal material. Material and Methods: The subjects were plaque sample of 20 children aged 7-12 years. Research begins by making toothpaste from ant nest extract. Samples of children's dental plaque were inserted into BHIB media, after which incubated for 24 hours, 1/10 dilution with BHIB media three times, followed by TYC media planting and incubation of anaerob with temperature 37 0 C for 48 hours. After that then count the number of colonies of S. mutans. Results: On ethyl acetate extract of ant nest incubated at room temperature with concentration 20%, 40%, 60%, 80%, 100% obtained a decrease from each treatment amount of Streptococcus mutans colony on TYC media with median value of each treatment was 89, 67, 64, 61, 59 and 51 for the ethyl acetate fraction, and 62, 61, 60, 59, 49 at the ethanol fraction. There was no significant difference between the six concentration groups (p>0.05). Conclusion: Flavonoids extract of ant nest plants have growth barrier on Streptococcus mutans bacteria, the greater the concentration given the greater the number of S. mutans colony.

Highlights

Dental caries is a problem in oral health in Indonesia with a prevalence of 90.05%, the high prevalence is due to the lack of public awareness of the importance of oral health
Samples of children's dental plaque were inserted into Brain Heart Infusion Broth (BHIB) media, after which incubated for 24 hours, 1/10 dilution with BHIB media three times, followed by Trypticase Yeast-Extract Cystine (TYC) media planting and incubation of anaerob with temperature 370C for 48 hours
On ethyl acetate extract of ant nest incubated at room temperature with concentration 20%, 40%, 60%, 80%, 100% obtained a decrease from each treatment amount of Streptococcus mutans colony on TYC media with median value of each treatment was 89, 67, 64, 61, 59 and 51 for the ethyl acetate fraction, and 62, 61, 60, 59, 49 at the ethanol fraction

Summary

Introduction

Dental caries is a problem in oral health in Indonesia with a prevalence of 90.05%, the high prevalence is due to the lack of public awareness of the importance of oral health. Ant nest plants with flavonoid content can inhibit the growth of oral microorganisms and bacterial activity by the enzyme Glucosyltransferase (GTFs). Streptococcus mutans colony is a collection of gram-positive bacteria, non-motile (immobile), facultative anaerobic bacteria It has the shape of a round or oval-shaped coccus, without spores, not encapsulated and arranged in rows.

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