Anti-Cancer Activity And Anti-Proliferation Ant Nests Flavonoid Fraction Test (Myrmecodya Pendans) Human Tongue Cancer Cells In Sp-C1

Abstract

Squamous cell carcinoma of the tongue is a malignant tumor derived from epithelial mucosa of the oral cavity and is largely a type of epidermoid carcinoma. The purpose of the study is to identify and analyze the effect of the flavonoid fraction of ant nests (Myrmecodia pendans) as an anti-cancer barrier against proliferation through Akt signaling and barrier mechanism of NF-KB in cancer cells SP-C1 of the tongue. The results of the study showed that the cytotoxicity test had the highest concentration of 1000 µg/ml to the lowest concentration of 7.8125 µg/ml in ethyl acetate fraction, ethanol, hexan fraction and water generates significant percentage data of the tongue cancer cell death SP-C1. Ethyl acetate flavonoid fraction in a concentration of 1000 µg/ml caused the percentage of cell death as much as 64.60%, and the lowest concentration of 7.8125 µg/ml led to cell death as much as 15.80 %. LC50 values of ethyl acetate fraction, ethanol fraction, hexan fraction and water fraction, respectively for 452.059 µg/ml; 937.562 µg/ml; 2691.535 µg/ml; 12302.69 µg/ml. Analysis antiproliferative flavonoid fraction and ethyl acetate fraction of ethanol based on concentration and incubation time to absorption optical density SP-C1 cells was statistically highly significant (p=0.00). Squamous cell carcinoma is the most common type of cancer that occurs in the oral cavity which is about 90-95% of total malignancy in the oral cavity. Location squamous cell carcinoma of the oral cavity is usually located on the tongue (ventral and lateral), lips, floor of the mouth, buccal mucosa, and retromolar area. (3) Squamous cell carcinoma of the tongue is a malignant tumor derived from epithelial mucosa of the oral cavity and is largely a type of epidermoid carcinoma. Squamous cell carcinoma of the tongue ranged between 25 and 50% of all malignant cancers in the mouth. (4) Several studies have proven the efficacy of ant nests for the treatment of cancer, it was revealed after the ant nest herbs can be used as an alternative medicine breast cancer chemotherapy with minimal side effects. Treatment with ant nests traditional medicine does not take a lot of cost and has minimal side effects compared to chemotherapy which requires a lot of cost and have many side effects. (5) Chemical screening test ant nests plant (Myrmecodia pendans) shows that this plant contains the chemical compounds of the flavonoid and tannin. Many mechanisms of flavonoid that has been revealed, including carcinogen inactivation, antiproliferation, cell cycle inhibition, induction of apoptosis and differentiation, and inhibition of angiogenesis. Ant nests ability empirically for the treatment of various types of cancer or tumors, allegedly related to the flavonoid content of ant nests.