Abstract
Tumor markers are biomacromolecules existing in blood or tissue, which are associated with cancer and whose measurement is useful in clinical diagnosis. The electrochemical immunoassay has attracted extensive attention due to its specific advantages such as high sensitivity, low cost, and easy handling. Here, a novel and highly efficient streptavidin-functionalized nitrogen doped graphene platform was proposed for sensitive electrochemical immunoassay of tumor marker. The biofunctionalized-nitrogen doped graphene platform shows large reactive surface area and excellent biocompatibility. The capture antibody can be efficiently immobilized on the biosensing platform surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using carcinoembryonic antigen (CEA) as model analyte, the highly efficient electrochemical immunosensing shows a linear range from 0.02 to 12 ng/mL with a correlation coefficient of 0.9980. The limit of detection was calculated to be 0.01 ng/mL (S/N=3). This biofunctionalized-nitrogen doped graphene platform shows much better performance compared with the platform without nitrogen doped graphene (shown in Fig. 1). This work provides a promising application in the determination of various tumor markers.
Published Version
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