Strengths and Limitations of different Chromogenic Media for the Identification of <i>Candida</i> Species

Abstract

The treat ment of invasive candidiasis and other Candida infections with the appropriate antifungal agent is assisted by the identification of Candida isolates to the species level. Rapid and accurate methods of differentiation are therefore imperat ive if t reat ment is to be effective, particularly in HIV-positive patients and in pregnant mothers where intervention may be necessary to reduce the risk for preterm delivery. The time used for isolation, identificat ion and detection of mixed cultures may be reduced with the help of available chro mogenic med ia. In this study, five commercial chro mogenic med ia were evaluated for the differentiation of Candida species. Six type-strains of Candida species were streaked onto each of five different chro mogenic media and incubated for up to 4 days at the different temperatures recommended by the manufacturers. This co mparative evaluation demonstrated the strengths and weaknesses of each mediu m employed and found CHROMagar™ Candida and Chro mogenic Candida Agar to be the most effective for distinguishing between different Candida species.