Abstract
In this comprehensive study, analytical restricted access media (RAM) columns were investigated to determine their performance in decreasing the humic acid interference encountered in the trace analysis of acidic herbicides in environmental water samples when employing reversed-phase liquid chromatography with UV detection (RPLC-UV). The selected analytical column RAM materials are 5-μm Semi-Permeable-Surface (SPS, Regis), 5-μm Internal Surface Reversed Phase (ISRP, Pinkerton, Regis), and 5-μm Hisep (Supelco); a precolumn containing 25-μm alkyl-diol-silica (ADS) RAM material (Merck) was also included in this study. Different LC configurations were investigated, including the single RAM column mode and column switching (LC/LC), employing one RAM column in combination with an analytical C18 column, or two RAM columns. Concerning methodology, both the single-residue method (SRM) approach and the multiresidue method (MRM) approaches were tested by analyzing SPE C18 extracts of reference water samples spiked w...
Published Version
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