Storage strategy for shale gas flowback water based on non-bactericide microorganism control

Abstract

Shale gas is a promising unconventional natural gas in the world, however the produced flowback water have severe challenges to surrounding water resource. Conventional reuse technology uses bactericide to control corrosive microorganism, which might bring uncontrolled drug resistance and other secondary pollution. In this study, storage strategy of flowback water was designed as a pre-control stage to decline corrosive microorganism. Dissolved oxygen and temperature were chosen as two key parameters based on microbial physiological and biochemical characteristics. Results showed that under the cross effect of temperature and dissolved oxygen, 15 °C and anaerobic condition had the optimal microorganism control effectiveness. Microorganism amount and live/dead cell ratio decreased by 63.7% and 68.74% respectively compared raw water. COD removal efficiency reduced to only 20%, indicating that the microorganism activity was extremely inhibited. However, microorganism in flowback water was more sensitive to dissolved oxygen compared to temperature. Redundancy analysis confirmed that dissolved oxygen contribution was as high as 91.5% while temperature was not significant (p > 0.05), the contribution rate was only 8.5%. Thermococcus, Archaeoglobus, Thermovirga, Thermotoga and Moorella were the dominated thermophilic, anaerobic and sulfate reduction or metal corrosion microorganism in flowback water, so all these identified microorganisms were control targets. Importantly, all the target microorganisms detected in flowback water were declined after different storage strategies. This study provides an effective storage strategy for flowback water to inhibit the microbial amount and activity without biocides addition, which could help promote the green exploitation of shale gas.