Storage of Platelets in a New Plastic Container

Abstract

The effect of storage of platelets in a new polyvinyl chloride (PVC) plastic material with a butyryl-n-trihexyl citrate (BTHC) plasticizer (PL 2209) was evaluated. The PL 1240 container, i. e. PVC plastic with a different plasticizer, tri-(ethylhexyl)-tri-mellitate, was used as a reference. Measurements of pH, pO(2), pCO(2), glucose, lactate, adenosine triphosphate, total adenine nucleotide content, lactate dehydrogenase and platelet factor 4 (PF4) were made during 5 days of storage. Similar results were noted comparing PL 2209 and PL 1240. Differences in pO(2) and pCO(2) indicate greater gas permeability in PL 2209 than in PL 1240. Significantly higher PF4 levels were found in PL 2209, but the difference could not be attributed to the PL 2209 container itself. Paired autologous reinfusion studies (111lndium) of 6 normal donors gave mean recovery values after 5-day storage of 41.1 ±7.4% (PL 2209) and 45.5 ±7.7% (PL 1240), t(1/2) 66±13 and 75±5h, survival time (linear model) 6.3 ±1.0 and 6.8 ±0.7 and survival time (multiple-hit model) 6.0 ±0.7 and 6.5 ±0.4 days, respectively. Only the difference in survival time (multiple-hit) was significantly higher in PL 1240. The corrected count increments at 12-24 h following transfusion were 13,300 ± 10,800 (PL 2209) and 13,600 ± 11,600 (PL 1240) with no statistically significant difference found. These results indicate PL 2209 as an equivalent alternative to PL 1240 for the 5-day storage of platelets.