Abstract
The cold storage potential of seeds and dormant buds for the preservation of forest tree germplasm was investigated. Seeds from European beech (Fagots sylratica), hybrid aspen (Populus tremula x P. tremuloides), larch (Larix decidua), Norway spruce (Picea abies), Scots pine (Pinus sylvestris), and silver fir (Abies alba) were stored for up to six days in liquid nitrogen (−196°C). Seed germination data indicated that in five of the six forest tree species, there was practically no loss of viability in the seeds stored in liquid nitrogen, as compared to controls. Beech seeds did not germinate following storage at −196°C. We are employing tissue culture technology to rescue beech embryo/cotyledon from seed stored in liquid nitrogen.
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