Abstract
1. 1. The stability of hepatic microsomal cytochrome P-450 monooxygenase and UDP glucuronyl transferase activities and cytosolic glutathione S-transferase activity in rainbow trout was investigated in order to develop a standardized freezing and storage procedure of liver samples that maintain the enzyme activity levels. 2. 2. Livers can be frozen and stored in liquid nitrogen a short period of time (3 days) without any significant loss of the biotransformation enzyme activities studied whereas freezing of the livers on dry ice resulted in a rapid loss of cytochrome P-450 activities. 3. 3. Cytochrome P-450 and UDP glucuronyl transferase activities in microsomes suspended in buffer containing 20% glycerol were stable for 1 year when the microsomes were stored at −80°C.
Published Version
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