Abstract
Stone pine (Pinus pinea L.) is a Mediterranean native conifer species. It is used for protection against soil erosion, ecosystem restoration and farmland afforestation. The most important commercial application is the production of timber and pine nuts. The stone pine kernels are highly appreciated as part of the Mediterranean diet because of their high nutritional value. They are currently one of the most expensive dried fruits. Breeding programs of stone pine mainly focus on the improvement of cone production by planting selected genotypes in grafted orchards. Therefore the production of clonal rootstocks is desirable. This chapter describes protocols to regenerate stone pine plants by somatic embryogenesis from immature zygotic embryos, and for the cryopreservation of embryogenic lines. Protocols used in the attemps to induce somatic embryogenesis in tissues from adult trees are also described.
Published Version
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